||The main purposes of this research are to develop integrated aquaculture techniques of multi-temperature and multi-nutrition gradients, to promote low-temperature property in local aquaculture industry, and to assist farmers to establish basic information of suitable environment for the progress of aquaculture industry. During the research period, the aquaculture module, which has the characteristics of \\\"one water resource, multi-stage usage, low-temperature before high-temperature, and aquacultural fish and shellfish interacting with algae,\\\" is constructed to develop integrated aquaculture techniques of multi-temperature and multi-nutrition gradients. It involves module organization for the design of research module, commercial-module industrial buildings, in-house farms, outdoor farms and other culture areas. Also, the module of LNG cool water (CPC Corporation) for multi-temperature gradients as well as water safety monitoring is constructed. Performing the cultural tests of module of LNG cool water for multi-temperature gradients in Apostichopus japonicas, Haliotis discus hannai, Epinephelus brunneus, Epinephelus lanceolatus, Penaeus monodon, Penaeus japonicas and Agardhiella subulata can reach 100% of survival rate. Enteromorpha intestinalis and Sarcodiaceylanica can reach 1% of growth rate. Assessment and investigation for the safety of water resource by LNG cool water had also been carried out. Each culture unit is suggested to culture high-value species for multi-temperature and multi-nutrition aquaculture system: there are eighteen species of fish and shellfish and ten types of algae, and their basic introduction, culture environments, culture methods, and the benefits of using deep-sea water with LNG cool water (CPC corporation) in aquaculture are described. Investigation of the proper high value species of algae has successfully led to reproduce fourteen different types of algae from intertidal zones around Taiwan, and establish critical techniques for culturing ten kinds of algae. Promote the efficiency of production, the economic benefits and pattern of management for culturing different high value of algae species in multi-temperature and multi-nutrition gradients. Analyzing the risk factors of reproduction processes and formulating the principles and standards for culturing have been established in multi-temperature and multi-nutrition gradients.